Gangliosides Are Essential for Bovine Adeno-Associated Virus Entry
نویسندگان
چکیده
منابع مشابه
Infectious entry pathway of adeno-associated virus and adeno-associated virus vectors.
We have investigated the infectious entry pathway of adeno-associated virus (AAV) and recombinant AAV vectors by assessing AAV-mediated gene transfer and by covalently conjugating fluorophores to AAV and monitoring entry by fluorescence microscopy. We examined AAV entry in HeLa cells and in HeLa cell lines which inducibly expressed a dominant interfering mutant of dynamin. The data demonstrate ...
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To better understand the relationship between primate adeno-associated viruses (AAVs) and those of other mammals, we have cloned and sequenced the genome of an AAV found as a contaminant in two isolates of bovine adenovirus that was reported to be serologically distinct from primate AAVs. The bovine AAV (BAAV) genome has 4,693 bp, and its organization is similar to that of other AAV isolates. T...
متن کاملExpression profiles of bovine adeno-associated virus and avian adeno-associated virus display significant similarity to that of adeno-associated virus type 5.
We present the first detailed expression profiles of nonprimate-derived adeno-associated viruses, namely, bovine adeno-associated virus (B-AAV) and avian adeno-associated virus (A-AAV), which were obtained after the infection of cell lines derived from their natural hosts. In general, the profiles of B-AAV and A-AAV were quite similar to that of AAV5; however, both exhibited features found for ...
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Adeno-Associated Virus Type 2 Contains an Integrin 5 1 Binding Domain Essential for Viral Cell Entry Aravind Asokan, Julie B. Hamra, Lakshmanan Govindasamy, Mavis Agbandje-McKenna, and Richard J. Samulski* Gene Therapy Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, and Department of Biochemistry and Molecular Biology, College of Medicine, University of F...
متن کاملAdeno-associated virus biology.
Adeno-associated virus (AAV) was first discovered as a contaminant of adenovirus stocks in the 1960s. The development of recombinant AAV vectors (rAAV) was facilitated by early studies that generated infectious molecular clones, determined the sequence of the genome, and defined the genetic elements of the virus. The refinement of methods and protocols for the production and application of rAAV...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 2006
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.02393-05